منابع مشابه
Methyl Green
1. Methyl green ("ethyl green") C. I. Number 685 was examined and found to behave identically with methyl green C. I. Number 684 (no longer available) in respect to molar extinction coefficient, effect of combination with polymerized DNA, failure to react with depolymerized DNA, and effect of pH. 2. The mass law permits the calculation of P/dye. This is found to be 13 P/dye. The same value is o...
متن کاملMethyl Green-pyronin
Study of the stoichiometry of the DNA-methyl green reaction by dialysis, precipitation of stain-nucleic acid mixtures, and the staining of nuclei of known DNA content, indicate that the compound consists of one dye molecule per 10 P. The significance of this result was discussed in the preceding paper (1). Histone and lanthanum (and probably other multivalent cations (3)) compete with the dye f...
متن کاملThe use of methyl green as a histochemical reagent
The cation of methyl green carries two positive charges, that of malachite green only one; but the two dyes behave towards tissue-constituents in almost exactly the same way. These dyes are not specific for chromatin. They colour certain objects that are devoid of DNA, even when they are used in very dilute solution. The granules of cells called Kornchenzellen in the connective tissue of the co...
متن کاملOn the Nature of the Deoxyribonucleic Acid-Methyl Green Reaction
Methyl green has frequently been used for histochemical and in vitro demonstration of deoxyribonucleic acid (DNA). I t has been reported that this color or staining reaction requires the presence of highly polymeric DNA (1) and that its molecular size influences the extent of the reaction. Thus, as the DNA is broken down by deoxyribonuclease (DNase), there is a corresponding decrease in the bin...
متن کاملMethyl green-pyronin stain for the diagnosis of trachoma.
The clinical diagnosis of TRIC-agent infection of the conjunctiva is not difficult for ophthalmologists in endemic areas such as Egypt, but the similarity of the disease to other types of follicular conjunctivitis makes laboratory investigations especially important (Thygeson, I960; Duke-Elder, I965). These include the staining of conjunctival scrapings with Lugol's iodine or Giemsa stain for d...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1950
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.34.2.147